<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestnikskfu</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Северо-Кавказского федерального университета</journal-title><trans-title-group xml:lang="en"><trans-title>Newsletter of North-Caucasus Federal University</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2307-907X</issn><publisher><publisher-name>North-Caucasus Federal University</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestnikskfu-514</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ТЕХНИЧЕСКИЕ НАУКИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>TECHNICAL SCIENCES</subject></subj-group></article-categories><title-group><article-title>АНАЛИЗ СОВРЕМЕННЫХ МЕТОДОВ ФРАКЦИОНИРОВАНИЯ БЕЛКОВ МОЛОЧНОГО СЫРЬЯ</article-title><trans-title-group xml:lang="en"><trans-title>ANALYSIS OF MODERN METHODS OF MILK PROTEIN FRACTIONATION</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Метель</surname><given-names>Владимир Сергеевич</given-names></name><name name-style="western" xml:lang="en"><surname>Metel'</surname><given-names>Vladimir</given-names></name></name-alternatives><email xlink:type="simple">metel@mokostav.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Куликова</surname><given-names>Ирина Кирилловна</given-names></name><name name-style="western" xml:lang="en"><surname>Kulikova</surname><given-names>Irina</given-names></name></name-alternatives><email xlink:type="simple">kik-st@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Анисимов</surname><given-names>Георгий Сергеевич</given-names></name><name name-style="western" xml:lang="en"><surname>Anisimov</surname><given-names>Georgij</given-names></name></name-alternatives><email xlink:type="simple">metel@mokostav.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Северо-Кавказский федеральный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>North-Caucasus Federal University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>05</day><month>03</month><year>2022</year></pub-date><volume>0</volume><issue>2</issue><fpage>27</fpage><lpage>32</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Метель В.С., Куликова И.К., Анисимов Г.С., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Метель В.С., Куликова И.К., Анисимов Г.С.</copyright-holder><copyright-holder xml:lang="en">Metel' V., Kulikova I., Anisimov G.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestnikskfu.elpub.ru/jour/article/view/514">https://vestnikskfu.elpub.ru/jour/article/view/514</self-uri><abstract><p>В статье рассмотрены методы фракционирования различных видов молочных белков: хроматография (ионообменная, аффинная и гидрофобная хроматография), мембранные методы и комбинированные методы. В последнее время были внедрены новые перспективные методы, такие как водное двухфазное разделение (ATPS) и магнитная ловушка. В этой статье рассматривается использование этих методов вместе с оценкой их эффективности в отношении выхода и чистоты основных белков. Также рассмотрены перспективные методы разделения казеинов в связи с растущим интересом к очищенным фракциям казеина и их пептидов.</p></abstract><trans-abstract xml:lang="en"><p>The methods of milk protein fractionation are analised: chromatography (ion exchange, affinity and hydrophobic chromatography), membrane methods and combined methods. The new prospective methods have been discribed, such as aqueous two-phase separation (ATPS) and magnetic fishing. The combination of the methods together have been estimated from a perspective of their effectiveness: yield and purity of the major proteins. The prospective methods of casein separation are also considered in considering with the growing interest in the high puriied fractions of casein and its peptides.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>молоко</kwd><kwd>казеин</kwd><kwd>сывороточные белки</kwd><kwd>хроматография</kwd><kwd>мембранные методы</kwd><kwd>milk</kwd><kwd>casein</kwd><kwd>whey proteins</kwd><kwd>chromatography</kwd><kwd>membrane methods</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">McSweeney P. L. H., Fox P. F. (eds.). Advanced dairy chemistry: volume 1A: proteins: basic aspects. 4th Edition. Springer Science. New York, 2013</mixed-citation><mixed-citation xml:lang="en">McSweeney P. L. H., Fox P. F. (eds.). Advanced dairy chemistry: volume 1A: proteins: basic aspects. 4th Edition. Springer Science. New York, 2013</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Jenness R., Holt C. Casein and lactose concentrations in milk of 31 species are negatively correlated. Experentia. 1987. Vol. 43. Issue 9. Рр. 1015-1018</mixed-citation><mixed-citation xml:lang="en">Jenness R., Holt C. Casein and lactose concentrations in milk of 31 species are negatively correlated. Experentia. 1987. Vol. 43. Issue 9. Рр. 1015-1018</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Mercier J. C., Grosclaude F., Ribadeau Dumas B. Structure primaire de la caseine aS1 bovine. Sequence complete. 1971. Eur. J. Biochem. Vol. 23. Рр. 41-51.</mixed-citation><mixed-citation xml:lang="en">Mercier J. C., Grosclaude F., Ribadeau Dumas B. Structure primaire de la caseine aS1 bovine. Sequence complete. 1971. Eur. J. Biochem. Vol. 23. Рр. 41-51.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Grosclaude F., Mahe M. F. and Ribadeau Dumas B. Structure primaire de la caseine аS1 et de la caseine в -bovine. 1973. Eur. J. Biochem. Vol. 40. Рр. 323-324.</mixed-citation><mixed-citation xml:lang="en">Grosclaude F., Mahe M. F. and Ribadeau Dumas B. Structure primaire de la caseine аS1 et de la caseine в -bovine. 1973. Eur. J. Biochem. Vol. 40. Рр. 323-324.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Farrell H. M., Jr., Malin E. L., Brown E. M., Mora-Gutierrez A. Review of the chemistry of аS2-casein and the generation of a homologous molecular model to explain its properties. 2009. J. Dairy Sci. Vol. 92. Рр. 1338-1353.</mixed-citation><mixed-citation xml:lang="en">Farrell H. M., Jr., Malin E. L., Brown E. M., Mora-Gutierrez A. Review of the chemistry of аS2-casein and the generation of a homologous molecular model to explain its properties. 2009. J. Dairy Sci. Vol. 92. Рр. 1338-1353.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Ribadeau Dumas B., Brignon G., Grosclaude F., Mercier, J. C. Structure primaire de la caseine в bovine. 1972. Eur. J. Biochem. Vol. 25. Рр. 505-514.</mixed-citation><mixed-citation xml:lang="en">Ribadeau Dumas B., Brignon G., Grosclaude F., Mercier, J. C. Structure primaire de la caseine в bovine. 1972. Eur. J. Biochem. Vol. 25. Рр. 505-514.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Minkiewicz Р., Slangen C. J., Lagerwerf F. M., Haverkamp J., Rollema H. S., Visser S. Reversed-phase high-performance liquid chromatographic separation of bovine к-casein macropeptide and characterization of isolated fractions // Journal of Chromatography A. Vol. 743. Рр. 123-135.</mixed-citation><mixed-citation xml:lang="en">Minkiewicz Р., Slangen C. J., Lagerwerf F. M., Haverkamp J., Rollema H. S., Visser S. Reversed-phase high-performance liquid chromatographic separation of bovine к-casein macropeptide and characterization of isolated fractions // Journal of Chromatography A. Vol. 743. Рр. 123-135.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Mayyada M. H. El-Sayed, Howard A. Chase Trends in whey protein fractionation. 2011. Biotechnol Lett. Vol. 33. Р. 1501-1511.</mixed-citation><mixed-citation xml:lang="en">Mayyada M. H. El-Sayed, Howard A. Chase Trends in whey protein fractionation. 2011. Biotechnol Lett. Vol. 33. Р. 1501-1511.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Gurgel Р. V., Carbonell R. G., Swaisgood H. E. Fractionation of whey proteins with a hexapeptide ligand affinity resin. Bioseparation. 2000. Vol. 9. Рр. 385-392.</mixed-citation><mixed-citation xml:lang="en">Gurgel Р. V., Carbonell R. G., Swaisgood H. E. Fractionation of whey proteins with a hexapeptide ligand affinity resin. Bioseparation. 2000. Vol. 9. Рр. 385-392.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Vyas H. K., Izco J. M., Jimenez-Flores R. Scale-up of native beta-lactoglobulin affinity separation process. J Dairy Sci. 2002. Vol. 85(7). Рр. 1639-1645.</mixed-citation><mixed-citation xml:lang="en">Vyas H. K., Izco J. M., Jimenez-Flores R. Scale-up of native beta-lactoglobulin affinity separation process. J Dairy Sci. 2002. Vol. 85(7). Рр. 1639-1645.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Ounis W. B., Gauthier S. F., Turgeon S. L., Roufik S., Pouliot Y. Separation of minor protein components from whey protein isolates by heparin affinity chromatography. Int Dairy J. 2008. Vol. 18. Рр. 1043-1050.</mixed-citation><mixed-citation xml:lang="en">Ounis W. B., Gauthier S. F., Turgeon S. L., Roufik S., Pouliot Y. Separation of minor protein components from whey protein isolates by heparin affinity chromatography. Int Dairy J. 2008. Vol. 18. Рр. 1043-1050.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Flashner M., Ramsden H., Crane L. J. Separation of proteins by high-performance anion-exchange chromatography. J Anal Biochem. 1983. Vol. 135(2). Рр. 340-344.</mixed-citation><mixed-citation xml:lang="en">Flashner M., Ramsden H., Crane L. J. Separation of proteins by high-performance anion-exchange chromatography. J Anal Biochem. 1983. Vol. 135(2). Рр. 340-344.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Colby C. B., O'Neill B. K., Vaugham F., Middelberg A. Р. J. Simulation of compression effects during scaleup of a commercial ion-exchange process. Biotechnol Ркщ. 1996. Рр. 12-662.</mixed-citation><mixed-citation xml:lang="en">Colby C. B., O'Neill B. K., Vaugham F., Middelberg A. Р. J. Simulation of compression effects during scaleup of a commercial ion-exchange process. Biotechnol Ркщ. 1996. Рр. 12-662.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Carrere H., Bascoul A., Floquet Р., Wilhelm A. M., Delmas H. Whey proteins extraction by fluidized ion exchange chromatography: simpliied modelling and economical optimization. Chem Eng J Biochem Eng J. 1996. Vol. 64(3). Рр. 307-317.</mixed-citation><mixed-citation xml:lang="en">Carrere H., Bascoul A., Floquet Р., Wilhelm A. M., Delmas H. Whey proteins extraction by fluidized ion exchange chromatography: simpliied modelling and economical optimization. Chem Eng J Biochem Eng J. 1996. Vol. 64(3). Рр. 307-317.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Vogt S., Freitag R. Comparison of anion exchange and hydroxyapatite displacement chromatography for the isolation of whey proteins. J Chromatogr. 1997. Vol. 760. Рр. 125-137.</mixed-citation><mixed-citation xml:lang="en">Vogt S., Freitag R. Comparison of anion exchange and hydroxyapatite displacement chromatography for the isolation of whey proteins. J Chromatogr. 1997. Vol. 760. Рр. 125-137.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Qingdao L., Amaqeet B., Jing-Xu (Jesse) Zhu, Argyrios M. Continuous protein recovery from whey using liquid-solid circulating luidized bed ion-exchange extraction. Biotechnol bioeng. 2002. Vol. 78. Рр. 157-163.</mixed-citation><mixed-citation xml:lang="en">Qingdao L., Amaqeet B., Jing-Xu (Jesse) Zhu, Argyrios M. Continuous protein recovery from whey using liquid-solid circulating luidized bed ion-exchange extraction. Biotechnol bioeng. 2002. Vol. 78. Рр. 157-163.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Hahn R. Р., Schulz M., Schau Р. C., Jungbauer A. Bovine whey fractionation based on cation-exchange chromatography. Journal of Chromatography A. 1998. Vol. 795. Рр. 277-287.</mixed-citation><mixed-citation xml:lang="en">Hahn R. Р., Schulz M., Schau Р. C., Jungbauer A. Bovine whey fractionation based on cation-exchange chromatography. Journal of Chromatography A. 1998. Vol. 795. Рр. 277-287.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">El-Sayed M. H., Chase H. A. Purification of the two major proteins from whey concentrate using a cation-exchange selective adsorption process. J Biotechnol Prog. 2010. Vol. 26(1). Рр. 192-199.</mixed-citation><mixed-citation xml:lang="en">El-Sayed M. H., Chase H. A. Purification of the two major proteins from whey concentrate using a cation-exchange selective adsorption process. J Biotechnol Prog. 2010. Vol. 26(1). Рр. 192-199.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Andersson J., Mattiasson B. Simulated moving bed technology with a simplified approach for protein purification separation of lactoperoxidase and lactoferrin from whey protein concentrate. Journal of Chromatography A. 2006. Vol. 1107. Рр. 88-95.</mixed-citation><mixed-citation xml:lang="en">Andersson J., Mattiasson B. Simulated moving bed technology with a simplified approach for protein purification separation of lactoperoxidase and lactoferrin from whey protein concentrate. Journal of Chromatography A. 2006. Vol. 1107. Рр. 88-95.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Gerberding S. J., Byers C. H. Preparative ion-exchange chromatography of proteins from dairy whey // Journal of Chromatography A. 1998. Vol. 808. Рр. 141-151.</mixed-citation><mixed-citation xml:lang="en">Gerberding S. J., Byers C. H. Preparative ion-exchange chromatography of proteins from dairy whey // Journal of Chromatography A. 1998. Vol. 808. Рр. 141-151.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Cheang B., Zydney A. A two stage ultrafiltration process for fractionation of whey protein isolate // Journal of Membrane Science. 2004. Vol. 231. Рр. 156-167.</mixed-citation><mixed-citation xml:lang="en">Cheang B., Zydney A. A two stage ultrafiltration process for fractionation of whey protein isolate // Journal of Membrane Science. 2004. Vol. 231. Рр. 156-167.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Almecija M. C., Ibanez R., Guadix A., Guadix E. M. Effect of pH on the fractionation of whey proteins with a ceramic ultrailtration membrane // Journal of Membrane Science. 2007. Vol. 288. Рр. 28-35.</mixed-citation><mixed-citation xml:lang="en">Almecija M. C., Ibanez R., Guadix A., Guadix E. M. Effect of pH on the fractionation of whey proteins with a ceramic ultrailtration membrane // Journal of Membrane Science. 2007. Vol. 288. Рр. 28-35.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Muller A., Daufin G., Chaufer B. Ultrafiltration modes of operation for the separation of alpha-lactalbumin from acid casein whey // Journal of Membrane Science. 1999. Vol. 153. Рр. 9-21.</mixed-citation><mixed-citation xml:lang="en">Muller A., Daufin G., Chaufer B. Ultrafiltration modes of operation for the separation of alpha-lactalbumin from acid casein whey // Journal of Membrane Science. 1999. Vol. 153. Рр. 9-21.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Bazinet L., Ippersiel D., Mahdavi B. Fractionation of whey proteins by bipolar membrane electro-acidification // Innovat Food Sci Emerg Technol. 2004. Vol. 5. Рр. 17-25.</mixed-citation><mixed-citation xml:lang="en">Bazinet L., Ippersiel D., Mahdavi B. Fractionation of whey proteins by bipolar membrane electro-acidification // Innovat Food Sci Emerg Technol. 2004. Vol. 5. Рр. 17-25.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Ndiaye N., Pouliot Y., Saucier L., Beaulieu L., Bazinet L. Electroseparation of bovine lactoferrin from model and whey solutions. Sep Purif Technol. 2010. Vol. 74. Рр. 93-99</mixed-citation><mixed-citation xml:lang="en">Ndiaye N., Pouliot Y., Saucier L., Beaulieu L., Bazinet L. Electroseparation of bovine lactoferrin from model and whey solutions. Sep Purif Technol. 2010. Vol. 74. Рр. 93-99</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Doyen A., Roblet C., Beaulieu L., Saucier L., Pouliot Y., Bazinet L. Impact of water splitting phenomenon during electrodialysis with ultrailtration membranes on peptide selectivity and migration // Journal of Membrane Science. 2013. Vol. 428. Рр. 349-356</mixed-citation><mixed-citation xml:lang="en">Doyen A., Roblet C., Beaulieu L., Saucier L., Pouliot Y., Bazinet L. Impact of water splitting phenomenon during electrodialysis with ultrailtration membranes on peptide selectivity and migration // Journal of Membrane Science. 2013. Vol. 428. Рр. 349-356</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Firdaous L., Dhulster P., Amiot J., Doyen A., Lutin F., Vezina L. P., Bazinet L. Investigation of the large-scale bioseparation of an antihypertensive peptide from alfalfa white protein hydrolysate by an electromembrane process // Journal of Membrane Science. 2010. Vol. 355. P. 175-181</mixed-citation><mixed-citation xml:lang="en">Firdaous L., Dhulster P., Amiot J., Doyen A., Lutin F., Vezina L. P., Bazinet L. Investigation of the large-scale bioseparation of an antihypertensive peptide from alfalfa white protein hydrolysate by an electromembrane process // Journal of Membrane Science. 2010. Vol. 355. P. 175-181</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Noudou V. Y. K., Suwal S., Amiot J., Mikhaylin S., Beaulieu L., Bazinet L. Simultaneous electroseparation of anionic and cationic peptides: Impact of feed peptide concentration on migration rate, selectivity and relative energy consumption // Separation and Purification Technology. 2016. Vol. 157. Рр. 53-59</mixed-citation><mixed-citation xml:lang="en">Noudou V. Y. K., Suwal S., Amiot J., Mikhaylin S., Beaulieu L., Bazinet L. Simultaneous electroseparation of anionic and cationic peptides: Impact of feed peptide concentration on migration rate, selectivity and relative energy consumption // Separation and Purification Technology. 2016. Vol. 157. Рр. 53-59</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">El-Sayed M. H., Chase H. A. Single and two-component cation-exchange adsorption of the two pure major whey proteins // Journal of Chromatography A. 2009. Vol. 1216. Рр. 8705-8711.</mixed-citation><mixed-citation xml:lang="en">El-Sayed M. H., Chase H. A. Single and two-component cation-exchange adsorption of the two pure major whey proteins // Journal of Chromatography A. 2009. Vol. 1216. Рр. 8705-8711.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Rojas E. G., Coimbra J. S., Minim L. A., Zuniga A. D., Saraiva S. H., Minim V P. Size-exclusion chromatography applied to the purification of whey proteins from the polymeric and saline phases of aqueous two-phase systems. Proc. Biochem. 2004. Vol. 39. Рр. 1751-1759.</mixed-citation><mixed-citation xml:lang="en">Rojas E. G., Coimbra J. S., Minim L. A., Zuniga A. D., Saraiva S. H., Minim V P. Size-exclusion chromatography applied to the purification of whey proteins from the polymeric and saline phases of aqueous two-phase systems. Proc. Biochem. 2004. Vol. 39. Рр. 1751-1759.</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Holder A. Cross-flow electro membrane filtration for the fractionation of dairy-based functional peptides. Dissertation, Stuttgart, Germany: University of Hohenheim, Verlag Dr. Hut. 2014.</mixed-citation><mixed-citation xml:lang="en">Holder A. Cross-flow electro membrane filtration for the fractionation of dairy-based functional peptides. Dissertation, Stuttgart, Germany: University of Hohenheim, Verlag Dr. Hut. 2014.</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Post A. E., Hinrichs J. Large-scale isolation of food-grade в-casein. Milchwissenschaft. 2011. Vol. 66. Рр. 361-364.</mixed-citation><mixed-citation xml:lang="en">Post A. E., Hinrichs J. Large-scale isolation of food-grade в-casein. Milchwissenschaft. 2011. Vol. 66. Рр. 361-364.</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Dickinson E. Interfacial, emulsifying and foaming properties of milk proteins // Fox P. F., McSweeney P. L. H. (Eds.) Advanced dairy chemistry. Vol. 1. Proteins (3rd ed., Vol. 1, Рр. 1229-1260). New York, USA: Kluwer Academic / Plenum Publishers.</mixed-citation><mixed-citation xml:lang="en">Dickinson E. Interfacial, emulsifying and foaming properties of milk proteins // Fox P. F., McSweeney P. L. H. (Eds.) Advanced dairy chemistry. Vol. 1. Proteins (3rd ed., Vol. 1, Рр. 1229-1260). New York, USA: Kluwer Academic / Plenum Publishers.</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Korhonen H. J. Bioactive milk proteins and peptides: From science to functional 250 aP. Lications // Australian Journal of Dairy Technology. 2009. Vol. 64. Рр. 16-25.</mixed-citation><mixed-citation xml:lang="en">Korhonen H. J. Bioactive milk proteins and peptides: From science to functional 250 aP. Lications // Australian Journal of Dairy Technology. 2009. Vol. 64. Рр. 16-25.</mixed-citation></citation-alternatives></ref><ref id="cit35"><label>35</label><citation-alternatives><mixed-citation xml:lang="ru">Huppertz T. Chemistry of caseins // McSweeney P. L. H. &amp; Fox P. F. (Eds.) Advanced dairy chemistry. Vol. 1A. Proteins: Basic aspects (Рр. 135-160). New York, USA: Springer.</mixed-citation><mixed-citation xml:lang="en">Huppertz T. Chemistry of caseins // McSweeney P. L. H. &amp; Fox P. F. (Eds.) Advanced dairy chemistry. Vol. 1A. Proteins: Basic aspects (Рр. 135-160). New York, USA: Springer.</mixed-citation></citation-alternatives></ref><ref id="cit36"><label>36</label><citation-alternatives><mixed-citation xml:lang="ru">Kessler, A., Menendez-Aguirre, O., Hinrichs, J., Stubenrauch, C., &amp; Weiss, J. (2013). aS-Casein-PE6400 mixtures: A fluorescence study. Faraday Discussions. Vol. 166. Рр. 399-416.</mixed-citation><mixed-citation xml:lang="en">Kessler, A., Menendez-Aguirre, O., Hinrichs, J., Stubenrauch, C., &amp; Weiss, J. (2013). aS-Casein-PE6400 mixtures: A fluorescence study. Faraday Discussions. Vol. 166. Рр. 399-416.</mixed-citation></citation-alternatives></ref><ref id="cit37"><label>37</label><citation-alternatives><mixed-citation xml:lang="ru">Tolkach A., Kulozik U. Fractionation of whey proteins and caseinomacropeptide by means of enzymatic crosslinking and membrane separation techniques. Journal of Food Engineering. 2005. Vol. 67. Рр. 13-20.</mixed-citation><mixed-citation xml:lang="en">Tolkach A., Kulozik U. Fractionation of whey proteins and caseinomacropeptide by means of enzymatic crosslinking and membrane separation techniques. Journal of Food Engineering. 2005. Vol. 67. Рр. 13-20.</mixed-citation></citation-alternatives></ref><ref id="cit38"><label>38</label><citation-alternatives><mixed-citation xml:lang="ru">Cayot P., Courthaudon J. L., Lorient D. Purification of aS-, в-and к-caseins by batchwise ion-exchange separation. Journal of Dairy Research. 1992. Vol. 59. Рр. 551-556.</mixed-citation><mixed-citation xml:lang="en">Cayot P., Courthaudon J. L., Lorient D. Purification of aS-, в-and к-caseins by batchwise ion-exchange separation. Journal of Dairy Research. 1992. Vol. 59. Рр. 551-556.</mixed-citation></citation-alternatives></ref><ref id="cit39"><label>39</label><citation-alternatives><mixed-citation xml:lang="ru">Shapira A., Assaraf Y. G., Epstein, D., Livney, Y. D. в-Casein nanoparticles as an oral delivery system for chemotherapeutic drugs: Impact of drug structure and properties on co-assembly. Pharmaceutical Research. 2010. Vol. 27. Рр. 2175-2186.</mixed-citation><mixed-citation xml:lang="en">Shapira A., Assaraf Y. G., Epstein, D., Livney, Y. D. в-Casein nanoparticles as an oral delivery system for chemotherapeutic drugs: Impact of drug structure and properties on co-assembly. Pharmaceutical Research. 2010. Vol. 27. Рр. 2175-2186.</mixed-citation></citation-alternatives></ref><ref id="cit40"><label>40</label><citation-alternatives><mixed-citation xml:lang="ru">Atamer Z., Post A. E., Schubert T., Holder A., Boom R. M., Hinrichs J. Bovine в-casein: Isolation, properties and functionality. A review. International Dairy Journal. 2017. Vol. 66. Рр. 115-125.</mixed-citation><mixed-citation xml:lang="en">Atamer Z., Post A. E., Schubert T., Holder A., Boom R. M., Hinrichs J. Bovine в-casein: Isolation, properties and functionality. A review. International Dairy Journal. 2017. Vol. 66. Рр. 115-125.</mixed-citation></citation-alternatives></ref><ref id="cit41"><label>41</label><citation-alternatives><mixed-citation xml:lang="ru">Post A. E., Ebert M., Hinrichs J. в-Casein as a bioactive precursor - processing for purification. Australian Journal of Dairy Technology. 2009. Vol. 64. Рр. 84-88.</mixed-citation><mixed-citation xml:lang="en">Post A. E., Ebert M., Hinrichs J. в-Casein as a bioactive precursor - processing for purification. Australian Journal of Dairy Technology. 2009. Vol. 64. Рр. 84-88.</mixed-citation></citation-alternatives></ref><ref id="cit42"><label>42</label><citation-alternatives><mixed-citation xml:lang="ru">Law A. J. R., Leaver J. Methods of extracting casein fractions from milk and caseinates and production of novel products. WO Patent 2003003847. 2007.</mixed-citation><mixed-citation xml:lang="en">Law A. J. R., Leaver J. Methods of extracting casein fractions from milk and caseinates and production of novel products. WO Patent 2003003847. 2007.</mixed-citation></citation-alternatives></ref><ref id="cit43"><label>43</label><citation-alternatives><mixed-citation xml:lang="ru">O'Mahony J. A., Smith K. E., Lucey J. A. Purification of в-casein from milk. US Patent 20070104847 A1. 2007.</mixed-citation><mixed-citation xml:lang="en">O'Mahony J. A., Smith K. E., Lucey J. A. Purification of в-casein from milk. US Patent 20070104847 A1. 2007.</mixed-citation></citation-alternatives></ref><ref id="cit44"><label>44</label><citation-alternatives><mixed-citation xml:lang="ru">Christensen J., Holst H. H. Method of producing beta-casein compositions. EU patent EP2947996 B1. 2016.</mixed-citation><mixed-citation xml:lang="en">Christensen J., Holst H. H. Method of producing beta-casein compositions. EU patent EP2947996 B1. 2016.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
